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Advanced Imaging Techniques
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Structured Illumination Microscopy (SIM)
Utilizes patterned light to interact with the specimen, which is then processed to reconstruct an image with resolution beyond conventional limits.
Photoacoustic Imaging
Imaging technique combining optical and ultrasound methods by detecting sound waves emitted by tissues after absorbing pulsed laser light, providing insights into tissue's optical properties and structure.
Diffraction Phase Microscopy
A quantitative phase imaging technique that uses the phase shift that light undergoes as it passes through a transparent sample to construct detailed images about sample's optical path length changes.
X-ray Crystallography
A technique to determine the atomic and molecular structure of a crystal by directing X-ray beams at it and measuring the angles and intensities of the resulting diffractions, used to identify molecular structure.
Total Internal Reflection Fluorescence (TIRF) Microscopy
This method uses the phenomenon of total internal reflection to create an evanescent wave that excites fluorescent molecules in a very thin region near the glass-water interface, improving surface selectivity and reducing background noise.
Confocal Microscopy
A technique that uses point illumination and a spatial pinhole to eliminate out-of-focus light in specimens that are thicker than the focal plane, enhancing optical resolution and contrast.
Optical Coherence Tomography (OCT)
An imaging technique similar to ultrasound but using light instead of sound, providing high-resolution cross-sectional images of the retina, cornea, or other tissues.
Fourier Transform Infrared (FTIR) Spectroscopy
A technique that measures the absorbance of infrared radiation by a sample at different wavelengths, creating a spectrum characteristic of the sample's molecular composition and structure.
Stimulated Emission Depletion (STED) Microscopy
A technique that surpasses the diffraction limit of light to provide super-resolution images. It uses one laser beam to excite fluorescent dyes and another to deactivate fluorescence around the targeted focal spot.
Fluorescence Recovery After Photobleaching (FRAP)
A method used to study the movement and diffusion of molecules within live cells. It involves photobleaching a region of interest and monitoring the recovery of fluorescence in that area over time.
Two-Photon Excitation Microscopy
A fluorescence imaging technique that allows imaging of living tissue up to about one millimeter in thickness. It uses two photons of lower energy instead of one photon of higher energy to excite a fluorophore.
Digital Holographic Microscopy
An imaging technique that records the hologram of a sample with a digital sensor, then reconstructs the image computationally to visualize depth information and optical properties.
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