Discover published sets by community

A molecular tool used to make precise changes in the DNA of organisms, from bacteria to human cells.

An enzyme that cuts DNA at a specific location dictated by an RNA guide sequence.

A synthetic RNA molecule designed to guide Cas9 to a specific location in the genome where a cut is intended.

A short sequence of DNA recognized by Cas9 enzyme to initiate binding and create a double-strand break.

A DNA repair mechanism that often leads to insertions or deletions when used with CRISPR-Cas9, potentially knocking out a gene.

A precise DNA repair mechanism used in conjunction with CRISPR-Cas9 when introducing specific mutations or inserting new genetic material.

A genetic engineering process where CRISPR-Cas9 is used to disrupt the function of a gene, often by creating small insertions or deletions.

A process where CRISPR-Cas9 and HDR are used to insert a specific DNA sequence into a particular genomic location.

A genetic engineering technique that promotes the inheritance of a particular gene or trait to increase its prevalence in a population.

Unintended genetic modifications in non-target locations in the genome caused by CRISPR-Cas9 action.

The effectiveness with which CRISPR-Cas9 induces editing at the intended target site in the genome.

A class of CRISPR-based tools enabling the direct, irreversible conversion of one DNA base pair to another without introducing DNA breaks.

A versatile and precise genome editing technique that uses a Cas9 nickase and a specially engineered reverse transcriptase.

An alternative CRISPR protein to Cas9 that has distinct PAM requirements and produces staggered DNA cuts.