Logo
Pattern

Discover published sets by community

Explore tens of thousands of sets crafted by our community.

Genetic Engineering Techniques

20

Flashcards

0/20

Still learning
StarStarStarStar

CRISPR-Cas9

StarStarStarStar

A genome editing tool that allows for precise, directed changes to genomic DNA. CRISPR-Cas9 uses a guide RNA to direct the Cas9 enzyme to a specific sequence for cutting.

StarStarStarStar

Gel Electrophoresis

StarStarStarStar

A technique used to separate DNA, RNA, or proteins based on their size and charge, using an electric field through a gel matrix.

StarStarStarStar

Polymerase Chain Reaction (PCR)

StarStarStarStar

A method used to amplify a specific DNA segment. It involves cycles of heating and cooling that enable DNA denaturation, annealing of primers, and extension by a DNA polymerase.

StarStarStarStar

Southern Blotting

StarStarStarStar

A method for detection of a specific DNA sequence in DNA samples. DNA is fragmented, separated by electrophoresis, and then transferred to a membrane to be probed.

StarStarStarStar

Northern Blotting

StarStarStarStar

A technique to detect specific RNA sequences, where RNA is separated by gel electrophoresis, transferred to a membrane, and probed with labelled molecules.

StarStarStarStar

Genome-Wide Association Studies (GWAS)

StarStarStarStar

Studies that are conducted to find the association between specific genetic variants and particular diseases by scanning the genomes from many different people.

StarStarStarStar

Zinc Finger Nucleases (ZFNs)

StarStarStarStar

A type of engineered DNA-binding protein that facilitates targeted editing of the genome by creating double-strand breaks in DNA at user-specified locations.

StarStarStarStar

Gene Therapy

StarStarStarStar

A technique that aims to treat or prevent disease by modifying the genes within an individual's cells.

StarStarStarStar

Gene Knockout

StarStarStarStar

A genetic technique in which one of an organism's genes is made inoperative ('knocked out' of the genome).

StarStarStarStar

Sanger Sequencing

StarStarStarStar

A method of DNA sequencing based on the selective incorporation of chain-terminating dideoxynucleotides by DNA polymerase during in vitro DNA replication.

StarStarStarStar

Chromatin Immunoprecipitation (ChIP)

StarStarStarStar

A method used to determine the location of DNA binding sites on the genome for a particular protein of interest.

StarStarStarStar

Phage Display

StarStarStarStar

A lab technique used to study protein-protein, protein-peptide, and protein-DNA interactions by fusing the protein of interest to a bacteriophage coat protein.

StarStarStarStar

Gene Knock-in

StarStarStarStar

A genetic engineering technique that allows for the insertion of a gene at a particular locus. This is used to introduce a new gene or modify an endogenous gene.

StarStarStarStar

Recombinant DNA Technology

StarStarStarStar

A method used to join together DNA molecules from two different species and insert them into a host organism to produce new genetic combinations.

StarStarStarStar

Next-Generation Sequencing (NGS)

StarStarStarStar

A high-throughput DNA sequencing technology that has revolutionized genomics by allowing the sequencing of an entire human genome within a single day.

StarStarStarStar

Fluorescence in situ hybridization (FISH)

StarStarStarStar

A cytogenetic technique that uses fluorescent probes that bind to only those parts of the chromosome with a high degree of sequence complementarity.

StarStarStarStar

RNA Interference (RNAi)

StarStarStarStar

A biological process in which RNA molecules inhibit gene expression by causing the destruction of specific mRNA molecules.

StarStarStarStar

Homologous Recombination

StarStarStarStar

A type of genetic recombination in which nucleotide sequences are exchanged between two similar or identical molecules of DNA.

StarStarStarStar

Microarray Analysis

StarStarStarStar

A tool used to analyze gene expression by allowing researchers to examine large numbers of DNA probes simultaneously on a small chip.

StarStarStarStar

TALENs (Transcription Activator-Like Effector Nucleases)

StarStarStarStar

Genetic engineering tools that can cut specific sequences of DNA. They are made of a transcription activator-like effector DNA-binding domain fused to a DNA cleavage domain.

Know
0
Still learning
Click to flip
Know
0
Logo

© Hypatia.Tech. 2024 All rights reserved.