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Surgical Sterilization Procedures

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Peracetic Acid Sterilization

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Uses peracetic acid to oxidize and destroy microbial cell components. It is a low-temperature sterilization method for heat-sensitive items.

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Immersion Sterilization

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Dipping items into germicidal chemicals sufficient for sterilization. Mainly for items that cannot withstand heat sterilization.

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Radiation Sterilization (Gamma)

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Utilizes gamma rays to break down DNA of microorganisms. Often used for single-use medical supplies and pharmaceuticals.

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Boiling Water

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A simple, yet less effective method where items are immersed in boiling water for a period, primarily used in less critical situations.

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Autoclaving

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Procedure that uses steam under pressure to kill microorganisms. Commonly used to sterilize surgical instruments.

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Ethylene Oxide Sterilization

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A chemical sterilization method that uses ethylene oxide gas to disrupt DNA of microorganisms. Used for heat-sensitive materials.

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Pulsed Light Sterilization

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Involves short, intense pulses of broad-spectrum light to destroy microorganisms. Used for surface sterilization of packaging and equipment.

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Ultraviolet (UV) Radiation

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Exposure to UV light at specific wavelengths to disrupt DNA, often used for surface disinfection and air purification.

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Chemiclave Sterilization

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Uses a combination of chemicals in a pressurized chamber to sterilize equipment, similar to an autoclave but designed for chemical vapor.

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Microwave Sterilization

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Uses microwave radiation to heat water molecules, producing steam that sterilizes the item. Effective for some simple applications.

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Ultrasonic Cleaning

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Although not a sterilization technique, it's used to remove fine particles from items prior to sterilization. It relies on high-frequency sound waves.

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Chemical Sterilization with Glutaraldehyde

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A high-level disinfectant that, when used in higher concentrations and longer exposure times, can act as a sterilant. Frequently used for delicate surgical instruments.

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Ionizing Radiation Sterilization (Electron Beam)

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Sterilization achieved by using a high-energy electron beam that destroys microorganisms' DNA. Suitable for medical devices and labware.

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High-level Disinfection

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A process that kills most microorganisms but may not eliminate all spores. It is not a full sterilization method but used for items that cannot withstand standard sterilization.

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Gas Plasma Sterilization

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Plasma is generated from hydrogen peroxide to produce free radicals that kill microorganisms. Suitable for heat-sensitive and moisture-sensitive items.

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Steam Undervacuum Sterilization

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A method where steam is introduced into a vacuum to reach sterilization temperatures quickly, for items that cannot withstand autoclave pressures.

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Moist Heat Sterilization

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Uses hot water or steam at lower temperatures than autoclaving, suitable for some heat- and moisture-stable items.

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Filtration Sterilization

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Passes liquids or gases through filters with pore sizes small enough to remove microorganisms. Typically used for heat-sensitive liquids.

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Cold Sterilization

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Involves the use of liquid chemical sterilants at low temperatures for delicate instruments. It is a lengthy process that requires thorough rinsing.

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Dry Heat Sterilization

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Uses high temperature for an extended period to kill microorganisms, specifically for materials that can withstand high temperatures.

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