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Biochemical Pathways

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Urea Cycle

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Detoxification of ammonia to urea. Steps include carbamoyl phosphate formation, citrulline and arginine synthesis, and urea formation by arginase activity.

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Pentose Phosphate Pathway

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Produces ribose-5-phosphate for nucleotide synthesis and NADPH for reductive biosynthesis. Steps include glucose-6-phosphate dehydrogenation and ribulose-5-phosphate isomerization.

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Translation

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Protein synthesis from mRNA template in ribosomes. Steps include tRNA charging, initiation at the AUG start codon, elongation and peptide bond formation, and termination at a stop codon.

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DNA Replication

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Synthesis of a new DNA strand from an existing template strand. Steps involve helix unwinding by helicase, primer synthesis by primase, elongation by DNA polymerase, and joining fragments by ligase.

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Transcription

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Synthesis of RNA from a DNA template by RNA polymerase. Steps include initiation at the promoter, elongation of the RNA transcript, and termination.

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Glycolysis

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Conversion of glucose to pyruvate with ATP and NADH production. Steps include glucose phosphorylation, fructose-1,6-bisphosphate cleavage, and pyruvate formation.

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Gluconeogenesis

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Synthesis of glucose from non-carbohydrate precursors. Involved steps are pyruvate carboxylation, phosphoenolpyruvate formation, fructose-1,6-bisphosphatase step, and glucose-6-phosphatase step.

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Electron Transport Chain

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Transfer of electrons from NADH and FADH2 to oxygen, forming water, and the generation of a proton gradient used to produce ATP. Steps include electron transfer through complexes, creation of a proton gradient, and ATP synthesis.

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Beta-Oxidation of Fatty Acids

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Fatty acids are broken down into acetyl-CoA units, which enter the Krebs cycle, and produce NADH and FADH2. Steps include acyl-CoA formation, enoyl-CoA hydration, β-hydroxyacyl-CoA dehydrogenation, and acetyl-CoA thiolysis.

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Calvin Cycle

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Photosynthetic process of carbon fixation in plants; involves the conversion of CO2 into glucose. Steps include CO2 fixation by ribulose bisphosphate, reduction of 3-phosphoglycerate, and regeneration of ribulose bisphosphate.

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Lipid Biosynthesis

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Synthesis of fatty acids and triglycerides from acetyl-CoA. Steps include acetyl-CoA carboxylation to malonyl-CoA, fatty acid chain elongation, and esterification to glycerol backbone.

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Krebs Cycle (Citric Acid Cycle)

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Acetyl-CoA is oxidized to CO2 with ATP, NADH, and FADH2 production. Steps include citrate formation, isomerization to isocitrate, α-ketoglutarate and succinyl-CoA formation.

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