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Plant Genetic Engineering Basics

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Reporter Genes

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Genes that are attached to a regulatory sequence of interest and whose expression is used to monitor the activity of that sequence.

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Terminator Sequence

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A sequence of DNA that signals the end of gene transcription.

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Transgene

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A gene introduced into a plant that originates from a different species or is artificially constructed.

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Regeneration

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In plant biotechnology, the process by which a plant grows from a single cell or tissue piece back into a full plant.

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Ti Plasmid

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A tumor-inducing plasmid found in Agrobacterium tumefaciens, used as a vector for genetic engineering in plants.

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Somatic Embryogenesis

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A form of plant tissue culture where somatic (non-reproductive) cells are induced to develop into embryos that can then grow into plants.

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Selectable Marker Genes

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Genes that are inserted into plants to help identify cells that have been successfully transformed with new genetic material.

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Marker-Assisted Selection

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A method where markers linked to certain genes of interest are used to select plants that have desired traits, enhancing breeding programs.

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CRISPR-Cas9

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A genome editing tool that allows for the precise modification of DNA within organisms.

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Vector

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A carrier DNA molecule that can transport foreign genetic material into another cell, where it can be replicated and/or expressed.

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Plant Proprietary Protection

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The intellectual property rights specifically tailored to protect plant varieties and plant breeders' innovations.

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Gene Silencing

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A natural or engineered process where the expression of a specific gene is suppressed.

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Bioinformatics

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The use of computational tools to analyze and interpret biological data, especially genetic sequences.

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Agrobacterium tumefaciens

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A bacterium used as a tool for plant transformation; utilizes a naturally occurring plasmid to insert genetic material into plant cells.

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Floral Dip

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A transformation technique where flowering plants are dipped into a solution containing Agrobacterium to transfer new genetic material without tissue culture.

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Tissue Culture

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The technique of growing plants cells or tissues in an artificial medium separate from the parent organism.

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Genetic Transformation

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The process of introducing new DNA into a plant cell to change its characteristics.

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Promoter

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A DNA sequence that regulates the expression of a gene by providing a binding site for RNA polymerase.

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Gene Gun Method (Biolistics)

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A physical method of plant transformation where DNA is literally shot into plant cells using microscopic particles.

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RNA Interference (RNAi)

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A biological process where RNA molecules inhibit gene expression by neutralizing targeted mRNA molecules.

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