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Mass Spectrometry in Proteomics
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Tandem Mass Spectrometry (MS/MS)
Application: Sequencing of peptides and identification of post-translational modifications; Type of Information Obtained: Fragment ion patterns for sequence analysis
Matrix-Assisted Laser Desorption/Ionization (MALDI)
Application: Identifying and characterizing proteins; Type of Information Obtained: Protein/peptide mass, peptide mass fingerprinting
Surface-Enhanced Laser Desorption/Ionization (SELDI)
Application: Protein profiling and biomarker discovery; Type of Information Obtained: Mass-to-charge ratios of adsorbed proteins
Fourier Transform Ion Cyclotron Resonance (FT-ICR)
Application: High-resolution mass analysis; Type of Information Obtained: Accurate mass measurements, isotopic distribution
Time-of-Flight (TOF)
Application: Peptide mass fingerprinting for protein identification; Type of Information Obtained: Mass-to-charge ratio based on ion flight time
Electrospray Ionization (ESI)
Application: Protein and peptide quantification; Type of Information Obtained: Multiple charge state ions, protein/peptide sequence
Ion Mobility Spectrometry-Mass Spectrometry (IMS-MS)
Application: Structural analysis of proteins and complexes; Type of Information Obtained: Collisional cross-sections of ions, which can be related to structure
Orbitrap Mass Spectrometry
Application: High-throughput proteomics; Type of Information Obtained: High-resolution and accurate mass measurements
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