Discover published sets by community

Quality control in RNA-seq ensures that the sequence data are of high quality and free from technical errors, involving steps such as assessment of raw read quality and post-sequencing data cleaning.

RNA sequencing (RNA-seq) is a high-throughput sequencing technique used to determine the quantity and sequence of RNA in a sample at a given moment, capturing a snapshot of gene expression.

Expression quantification involves counting the number of reads that map to particular genes or transcripts to determine their levels of expression within the sample.

Read mapping is the process of aligning the sequences obtained from RNA-seq to a reference genome or transcriptome to determine their origin.

Isoform discovery in RNA-seq involves determining the different splice variants, or isoforms, of genes that may be expressed in a cell, contributing to its complexity.

Library preparation is the process of creating a collection of DNA fragments for sequencing; in RNA-seq, RNA is first converted into a library of cDNA fragments.

Sequencing by synthesis is a common technique used in RNA-seq where DNA polymerase synthesizes a complementary strand of DNA, and the identity of each nucleotide is determined as it is incorporated.

Differential expression analysis is used in RNA-seq to compare gene expression levels between different samples, conditions, or time points to identify genes with significant changes in expression.

Reverse transcription is a technique used to convert RNA into complementary DNA (cDNA) during library preparation for RNA-seq, using the enzyme reverse transcriptase.