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Cloning vectors designed to propagate large inserts of DNA in bacteria, usually E. coli, with more stability than other bacterial vectors because of their low copy number.

A method used to randomly clone pieces of DNA which are then screened to find the desired sequence. Often used in sequencing genomes.

This cloning method utilizes the properties of topoisomerase I to join DNA fragments in a simple, one-step reaction that does not require DNA ligase.

Cloning of complementary DNA (cDNA) is a method used to clone the mRNA (messenger RNA). It involves reverse transcription of mRNA into cDNA.

This is a molecular biology method that is used to make specific and intentional changes to the DNA sequence of a gene and its products.

Cloning vectors derived from the DNA elements of yeast chromosomes that can replicate and segregate as artificial chromosomes in yeast cells.

This process involves combining DNA from different sources and inserting them into a host organism to produce new genetic combinations that are valuable for science, medicine, agriculture, and industry.

A set of experimental methods used to assemble recombinant DNA and to direct its replication within a host organism.

A molecular biology method that enables the transfer of DNA fragments between different cloning vectors through recombination.