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Enzymatic Biochemistry
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Amylase
Catalyzes the breakdown of starch into sugars in microbes.
Lipase
Involved in the degradation of lipids, converting them into glycerol and fatty acids.
Protease
Enzyme responsible for proteolysis; breaking down proteins into peptides or amino acids.
DNA Polymerase
Enzyme that facilitates the replication of DNA by adding nucleotides to the growing DNA strand.
RNA Polymerase
An enzyme that synthesizes RNA from a DNA template during transcription.
Nitrifying bacteria
Converts ammonia to nitrate compounds, primarily facilitated by Nitrosomonas and Nitrobacter.
Carbonic Anhydrase
Helps to rapidly convert carbon dioxide and water to bicarbonate and protons, maintaining acid-base balance.
Lactase
Hydrolyses lactose into glucose and galactose, particularly important in dairy product fermentation.
Hydrogenase
Catalyzes the reversible oxidation of molecular hydrogen.
Nitrogenase
Catalyzes nitrogen fixation, converting atmospheric nitrogen to bioavailable ammonia.
Pectinase
Breaks down pectin, a polysaccharide found in plant cell walls, commonly used in fruit juice extraction.
Cellulase
Catalyzes the decomposition of cellulose into glucose or shorter polysaccharides.
Xylanase
Degrades the polysaccharide xylan into xylose, used in paper and feed industries.
Alcohol Dehydrogenase
Catalyzes the conversion of alcohols to aldehydes or ketones.
Lactate Dehydrogenase
Catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+.
Hexokinase
Phosphorylates hexoses to form hexose phosphate, a key step in glycolysis.
Acetate Kinase
Catalyzes the conversion of acetate to acetyl-CoA, an important step in many fermentative pathways.
Superoxide Dismutase
Protects cells against superoxide radical damage by catalyzing its conversion to oxygen and hydrogen peroxide.
Catalase
Breaks down hydrogen peroxide into water and oxygen, protecting cells from oxidative damage.
Urease
Hydrolyzes urea to form ammonia and carbon dioxide, aiding in nitrogen recycling.
Glucoamylase
Breaks down starch into glucose, thereby completing the digestion of polysaccharides.
Glucose-6-Phosphate Dehydrogenase
Catalyzes the first step in the pentose phosphate pathway, generating NADPH and pentoses.
Phosphofructokinase
Regulates the rate of glycolysis by converting fructose-6-phosphate to fructose-1,6-bisphosphate.
Pyruvate Kinase
Catalyzes the final step of glycolysis, converting PEP to pyruvate and generating ATP.
Isocitrate Dehydrogenase
Catalyzes the oxidative decarboxylation of isocitrate, producing alpha-ketoglutarate and CO2 in the citric acid cycle.
Malate Dehydrogenase
Catalyzes the interconversion of malate to oxaloacetate and generates NADH in the TCA cycle.
Transaminase
Facilitates the conversion of amino acids to alpha-keto acids through transfer of an amino group.
Sulfite Reductase
Reduces sulfite to sulfide, a crucial step in microbial sulfate reduction.
Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase (RuBisCO)
Key enzyme in the Calvin cycle, catalyzing carbon dioxide assimilation in photosynthetic organisms.
Glutamine Synthetase
Catalyzes the synthesis of glutamine from glutamate and ammonia in the nitrogen assimilation process.
Arginase
Converts arginine into urea and ornithine, and plays a role in the urea cycle.
Acetyl-CoA Carboxylase
Regulatory enzyme in fatty acid biosynthesis that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA.
Pyruvate Dehydrogenase
Converts pyruvate to acetyl-CoA, linking glycolysis to the TCA cycle.
Beta-Galactosidase
Hydrolyzes the disaccharide lactose into monosaccharides glucose and galactose during lactose metabolism.
Coenzyme Q – Cytochrome C Reductase
Part of the electron transport chain, it transfers electrons from Coenzyme Q to cytochrome c.
Peroxisomal Oxidase
Oxidizes fatty acids in peroxisomes, generating hydrogen peroxide.
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