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Microbial Growth and Nutrition
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Lag Phase
The lag phase is a period of adaptation before growth. Microbes are adjusting to their environment and synthesizing essential cellular molecules.
Exponential (Log) Phase
During the exponential phase, microbial populations undergo rapid, constant growth, doubling at regular intervals.
Stationary Phase
Growth rate slows as resources are exhausted and waste accumulates, leading to a balance between cell division and death.
Death Phase
Microbial population experiences a decline in number as cells die off at a consistent rate due to the lack of nutrients and accumulation of toxic products.
Carbohydrates
Carbohydrates are primary energy sources for many microbes and are metabolized through various pathways like glycolysis and fermentation.
Proteins
Proteins can serve as both an energy source and a supply of amino acids for microbial growth and enzyme synthesis.
Lipids
Lipids are used by microbes as an energy reserve and structural components of cell membranes through β-oxidation and other processes.
Nitrogen
Nitrogen is essential for the synthesis of amino acids, nucleic acids, and other cell constituents; microbes may fix atmospheric nitrogen or assimilate it in organic or inorganic forms.
Vitamins
Vitamins act as coenzymes or precursors for coenzymes and are crucial for many metabolic processes in microbes.
Oxygen Requirements
Microbial oxygen requirements categorize microbes into groups like aerobes, anaerobes, microaerophiles, and facultative anaerobes, reflecting their energy generation strategies.
pH Tolerance
Microbes have specific pH ranges for growth, with acidophiles, neutrophiles, and alkaliphiles thriving in different pH environments.
Osmotic Pressure
Microbes vary in their requirements and tolerance for osmotic pressure; halophiles, for instance, thrive in high salt concentrations.
Temperature Range
Microbial growth rates vary with temperature; categories include psychrophiles, mesophiles, thermophiles, and hyperthermophiles.
Trace Elements
Trace elements are required in small amounts for enzyme function and maintenance of protein structure in microbes.
Phases of Microbial Growth Curve
The typical microbial growth curve includes lag, exponential (log), stationary, and death phases; each phase provides insight into microbial population dynamics.
Binary Fission
Binary fission is the primary mode of reproduction in bacteria, involving DNA replication, septum formation, and cell division.
Biofilms
Biofilms are complex communities of microorganisms attached to surfaces, protected by an extracellular matrix, and exhibit different characteristics than planktonic cells.
Quorum Sensing
Quorum sensing is a cell-density dependent regulation mechanism in bacteria, involving communication through signaling molecules, coordinating group behaviors like biofilm formation.
Aerobic Respiration
Aerobic respiration is a metabolic process in which microbes use oxygen as the final electron acceptor in the electron transport chain to generate ATP.
Anaerobic Respiration
Anaerobic respiration allows microbes to generate energy without oxygen, using alternative electron acceptors such as nitrates, sulfates, or carbon dioxide in the electron transport chain.
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