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Types of Microscopy
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Scanning Electron Microscopy (SEM)
Scans a beam of electrons over the specimen's surface. Used for detailed three-dimensional surface imaging at high magnifications.
Darkfield Microscopy
Features a dark background with a bright specimen. Useful for observing living, unstained cells.
Fluorescence Microscopy
Uses high-intensity light to excite fluorescent molecules in the sample. Commonly used in molecular biology to observe tagged molecules.
Confocal Microscopy
Offers three-dimensional imaging by focusing a laser light on a single plane within a specimen. Frequently used for detailed localization of cellular components.
Phase-Contrast Microscopy
Enhances contrast in transparent and colorless objects by using phase shifts. Ideal for live cell imaging.
Super-Resolution Microscopy
Surpasses the diffraction limit of light, allowing for the observation of structures smaller than 200 nm. Used in in-depth cellular research.
Transmission Electron Microscopy (TEM)
Transmits electrons through a specimen, revealing cellular structures with nanometer resolution. Used for ultrastructural examinations of specimens.
Atomic Force Microscopy (AFM)
Images surfaces by measuring forces between a sharp probe and specimen. Useful for imaging live cells in water or biological molecules.
Brightfield Microscopy
Utilizes light to illuminate the sample. Often used for stained or fixed specimens.
DIC Microscopy
Differential Interference Contrast Microscopy uses polarized light to create a 3D-like image. It's useful for live, unstained samples.
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