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Types of Microscopy

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Brightfield Microscopy

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Utilizes light to illuminate the sample. Often used for stained or fixed specimens.

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Darkfield Microscopy

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Features a dark background with a bright specimen. Useful for observing living, unstained cells.

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Phase-Contrast Microscopy

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Enhances contrast in transparent and colorless objects by using phase shifts. Ideal for live cell imaging.

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Fluorescence Microscopy

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Uses high-intensity light to excite fluorescent molecules in the sample. Commonly used in molecular biology to observe tagged molecules.

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Confocal Microscopy

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Offers three-dimensional imaging by focusing a laser light on a single plane within a specimen. Frequently used for detailed localization of cellular components.

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Transmission Electron Microscopy (TEM)

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Transmits electrons through a specimen, revealing cellular structures with nanometer resolution. Used for ultrastructural examinations of specimens.

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Scanning Electron Microscopy (SEM)

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Scans a beam of electrons over the specimen's surface. Used for detailed three-dimensional surface imaging at high magnifications.

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Atomic Force Microscopy (AFM)

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Images surfaces by measuring forces between a sharp probe and specimen. Useful for imaging live cells in water or biological molecules.

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DIC Microscopy

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Differential Interference Contrast Microscopy uses polarized light to create a 3D-like image. It's useful for live, unstained samples.

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Super-Resolution Microscopy

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Surpasses the diffraction limit of light, allowing for the observation of structures smaller than 200 nm. Used in in-depth cellular research.

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