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Principles of Sterilization

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Autoclaving

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Uses pressurized steam to kill all microorganisms. Commonly used for medical tools, laboratory equipment, and waste.

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Ethylene Oxide Gas Sterilization

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Ethylene oxide is a gas that can sterilize at low temperatures, making it useful for heat-sensitive materials like plastics and electronics.

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Dry Heat Sterilization

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Employs high temperatures, usually 160-170°C, for a duration depending on the item. It's suitable for materials that can't be sterilized using moist heat.

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Plasma Sterilization

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Utilizes low-temperature plasma within a vacuum to generate reactive species that destroy microorganisms. Suited for medical instruments sensitive to heat and humidity.

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Chemical Sterilization

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Employs chemicals like formaldehyde, peracetic acid, or hydrogen peroxide to sterilize surfaces and instruments. Often used when heat-sensitive equipment is involved.

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Incineration

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Direct flame or high-temperature oven incinerates organic material, ensuring complete destruction of all microbial life. Typically used for contaminated waste.

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Ultraviolet (UV) Radiation

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UV-C light disrupts microbial DNA, thus preventing replication. Useful for surface sterilization and in HVAC systems.

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Ion Beam Sterilization

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Applies ionized particles to the sterilization target, disrupting cellular structures. Emerging technique potentially useful for pharmaceuticals and tissues.

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Filtration

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Separates microbes from liquids or air using a physical barrier. It's important for heat-labile solutions and clean room air.

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Microwave Sterilization

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Microwaves generate heat by agitating water molecules in the microbial cells, leading to their destruction. Best for liquids and for disinfection rather than sterilization.

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Pulsed Light Sterilization

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Uses high-intensity, broad-spectrum light pulses to destroy microorganisms on surfaces, packaging, and foods with minimal heat generation.

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Chlorine Dioxide Gas Sterilization

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Chlorine dioxide gas is a strong oxidizing agent that can be used for sterilization purposes, particularly for large-scale disinfection tasks like water treatment.

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Gas Plasma Sterilization

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Similar to plasma sterilization but utilizes RF energy to generate the plasma state. Effective for thermally sensitive equipment and widely used in hospitals.

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Moist Heat Pasteurization

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This milder form of sterilization uses temperatures below boiling to reduce levels of non-spore forming pathogens, primarily in food and beverage industry.

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Gamma Irradiation

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Uses high-energy gamma photons, usually sourced from Cobalt-60, to destroy microorganisms. Ideal for single-use medical supplies and packaging.

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