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Biomedical Imaging Techniques

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Gamma Camera Imaging

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Detects gamma ray emissions from the body after administration of a radioactive tracer, used in nuclear medicine to visualize and quantify organ function.

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X-ray Radiography

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Uses ionizing radiation to create images of internal structures. High-energy X-ray photons pass through the body and are absorbed at different rates by different tissues, creating a contrast image on a detector.

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Dark Field Microscopy

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Increases contrast in unstained samples by using scattered light. It makes visible the structures in a specimen that are not seen with standard light microscopes.

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Optical Coherence Tomography (OCT)

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Infrared light is used to take cross-sectional images by measuring the echo time delay and intensity of backscattered light, particularly useful in ophthalmology for imaging the retina.

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Fluoroscopy

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A continuous X-ray beam is passed through the body and captured on a fluorescent screen, allowing real-time moving images, typically used for guiding medical procedures.

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Near-Infrared Spectroscopy (NIRS)

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A noninvasive technique that uses near-infrared light to measure tissue oxygenation and blood flow, often used in brain imaging and muscle studies.

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Mammography

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A specialized X-ray imaging that uses low-dose ionizing radiation, specifically for breast tissue to detect abnormalities and tumors.

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Computed Tomography (CT)

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Operates on the same principle as X-ray but takes multiple cross-sectional images as the X-ray source and detector rotate around the body, which are then reconstructed into a 3D image by a computer.

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Single Photon Emission Computed Tomography (SPECT)

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Similar to PET but uses gamma-emitting radioisotopes. Rotating gamma cameras detect emitted photons and a computer constructs 3D images, providing functional information about organs and tissues.

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Diffusion Tensor Imaging (DTI)

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An MRI-based neuroimaging method that measures the diffusion of water molecules in tissue to map neural pathways and brain fiber tracts.

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Magnetoencephalography (MEG)

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Measures magnetic fields produced by neuronal activity in the brain to map brain function and can assist in localizing regions for neurosurgery.

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Nuclear Magnetic Resonance Spectroscopy (NMR)

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Not primarily an imaging technique but can be used to create images. It detects the interaction of nuclei in a magnetic field with radio waves, to determine molecular structure and concentration.

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Echocardiography

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An ultrasound technique specifically for the heart, measuring the direction and speed of blood flow and providing images of the heart's structure and function.

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Optical Projection Tomography (OPT)

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A microscopic imaging technique that generates 3D images from whole embryos or organs using visible light, which is more suitable for non-transparent or fluorescently labeled samples.

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Confocal Microscopy

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A laser-scanning technique that increases optical resolution and contrast by using a spatial pinhole. It allows viewing high-resolution images of live cells and tissues in slices.

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Electron Microscopy

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Uses a beam of electrons instead of light to create images of small structures. Due to the shorter wavelength of electrons, it provides a higher resolution, useful for detailed tissue and cell structure visualization.

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Magnetocardiography (MCG)

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A non-contact, non-invasive technique to measure the magnetic fields produced by electrical activity in the heart, giving insight into cardiac function and arrhythmias.

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Thermography

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Infrared imaging that measures radiation emitted from the skin surface to map body temperature; commonly used for detecting inflammatory diseases, circulatory problems, and breast cancer screenings.

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Endoscopy

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Involves insertion of a long, flexible tube with a camera into the body to visually examine internal organs in real-time; widely used in gastrointestinal and surgical procedures.

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Photoacoustic Imaging

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Combines optical and ultrasound imaging; pulsed laser light is absorbed by tissues, which generates ultrasonic waves, detected to form images, useful in cancer imaging and vascular mapping.

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Bone Densitometry

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Measures the density of bones using X-ray or CT technology to assess the risk of fractures and diagnose conditions such as osteoporosis.

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Ultrasound Imaging

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Employs high-frequency sound waves which are transmitted into the body. Echoes are reflected back and received by the transducer. The time and strength of the echoes determine the location and characteristics of structures inside the body.

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Functional MRI (fMRI)

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An MRI method that measures brain activity by detecting changes associated with blood flow, based on the fact that cerebral blood flow and neuronal activation are linked.

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Positron Emission Tomography (PET)

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Utilizes radioactive tracers which emit positrons. These positrons meet electrons, leading to annihilation and production of gamma rays that are detected to create a functional image of metabolic activity.

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Digital Subtraction Angiography (DSA)

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Fluoroscopy-based technique that involves the subtraction of pre- and post-contrast images. This highlights the vascular structures by removing the background and is often used for examining blood vessels.

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Magnetic Resonance Imaging (MRI)

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Uses a strong magnetic field and radio waves to excite hydrogen protons in the body. When these protons realign with the magnetic field, they emit signals that are detected and used to create an image.

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Intravital Microscopy

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A form of microscopy that allows for the visualization of living organisms at the microscopic level, providing real-time insights into cellular and molecular processes within their natural context.

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Optogenetics Imaging

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Uses light to control neurons that have been genetically modified to express light-sensitive ion channels, enabling the study of the functions of brain circuits and the control of animal behavior.

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Intravenous Ultrasound (IVUS)

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An ultrasound technique where the transducer is placed on a catheter and inserted into blood vessels to visualize the interior of blood vessels and provide guidance for stents.

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Bioluminescence Imaging

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Utilizes the light produced by chemical reactions in genetically modified organisms that express luciferase, for tracking gene expression, the progression of tumors, or the response to treatment in living organisms.

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