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Electrophysiology Fundamentals

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Action Potential

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A rapid depolarization and repolarization of the cell membrane voltage, essential for nerve impulse transmission and muscle contraction.

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Electrocardiogram (ECG or EKG)

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A record of the electrical activity of the heart over a period of time, used in the diagnosis of cardiac conditions.

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Patch Clamp

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A technique to study single or multiple ion channels in cells by recording the currents that flow through them.

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Voltage Clamp

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An experimental method to control the membrane potential of a cell while measuring the ionic currents that pass through the membrane.

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Nernst Equation

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Used to calculate the equilibrium potential for a particular ion based on the concentration gradient across the membrane:

E=RTFzln[ion]out[ion]in E = \frac{RT}{Fz} \ln \frac{[ion]_{out}}{[ion]_{in}}

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Electroencephalogram (EEG)

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A test that detects electrical activity in the brain using small electrodes attached to the scalp.

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Depolarization

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The process of reducing the resting membrane potential, typically by the influx of sodium ions into the neuron.

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Ion Channel

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A protein that allows specific ions to pass through it across the cell membrane, contributing to various cell functions like action potentials.

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Resting Membrane Potential

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The voltage difference across the membrane when a neuron is not transmitting a signal, typically between -60 to -80 mV in neurons.

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Absolute Refractory Period

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The period during and immediately following an action potential when a neuron cannot initiate another action potential regardless of the strength of the stimulus.

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Hodgkin-Huxley Model

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A mathematical model that describes how action potentials in neurons are initiated and propagated, based on voltage-gated ion channels.

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Goldman-Hodgkin-Katz Equation

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A formula used to predict membrane potential from the concentrations of multiple ions and their respective permeability:

Vm=RTFlnPK[K+]o+PNa[Na+]o+PCl[Cl]iPK[K+]i+PNa[Na+]i+PCl[Cl]o V_m = \frac{RT}{F} \ln \frac{P_{K}[K^+]_o + P_{Na}[Na^+]_o + P_{Cl}[Cl^-]_i}{P_{K}[K^+]_i + P_{Na}[Na^+]_i + P_{Cl}[Cl^-]_o}

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Magnetoencephalography (MEG)

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A non-invasive neuroimaging technique for mapping brain activity by recording magnetic fields produced by electrical currents occurring naturally in the brain.

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Myocardial Action Potential

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The specific type of action potential observed in cardiac muscle cells, characterized by a prolonged plateau phase due to prolonged calcium ion influx.

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Local Field Potential (LFP)

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The electric potential in the extracellular space around neurons, representing the summed electrical activity of a population of neurons.

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Intracellular Recording

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The measurement of the electrical potential inside a neuron, providing detailed information about the electrical activities of the cell.

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Pacemaker Potential

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The spontaneous gradual depolarization of the membrane potential that occurs in the cells of the sinoatrial node, leading to the initiation of the action potential that dictates the heart rate.

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Hyperpolarization

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An increase in the membrane potential of a cell, relative to the normal resting potential, often resulting from the efflux of potassium ions.

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Repolarization

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The process of restoring the resting membrane potential after depolarization, typically involving the efflux of potassium ions.

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Relative Refractory Period

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The period following the absolute refractory period when a neuron can initiate another action potential, but only by a stronger than usual stimulus.

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Cardiac Ablation

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A procedure that destroys areas of heart tissue that are causing cardiac arrhythmias.

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Biopotential Electrode

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A device that enables the detection of electrical activity in living tissue and the conversion of ionic currents to electronic ones.

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Transmembrane Potential

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The voltage across a cell's plasma membrane, critical for various cellular activities including cell signaling and muscle contraction.

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Optogenetics

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A biological technique which involves the use of light to control neurons that have been genetically modified to express light-sensitive ion channels.

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Cable Theory

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A mathematical model used to describe how electrical signals attenuate as they travel along passive structures like dendrites and axons.

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Impedance Plethysmography

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A non-invasive method to measure changes in volume within an organ or the whole body, often used to assess blood flow.

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Galvanotaxis

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The movement of cells or organisms in response to an electric field, playing a role in processes such as wound healing and cell migration.

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Excitation-Contraction Coupling

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The mechanism by which an action potential leads to muscle contraction, involving the influx of calcium and interaction with the contractile proteins.

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Electrophyisiological Mapping

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The use of electrophysiological techniques to delineate the electrical activity of the heart or brain, often to identify abnormal tissue responsible for arrhythmias.

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Cardiac Electrophysiology Study (EPS)

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An invasive test that studies the electrical conduction system of the heart to assess the potential for arrhythmic episodes.

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Microneurography

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A technique to record nerve impulses and nerve traffic in individual peripheral nerve fibers.

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Ion Selectivity

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The ability of ion channels to discriminate between different ions, allowing some to pass while blocking others.

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Neuroprosthetics

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Devices that substitute a motor, sensory, or cognitive modality that might have been damaged as a result of an injury or a disease.

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Cardiac Pacemaker

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An artificial device that uses electrical impulses to regulate the beating of the heart, replacing or regulating the function of the natural pacemaker.

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