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Chromatography Techniques
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Flashcards
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Paper Chromatography
Purpose is to separate small amounts of substances based on their solubility; application includes teaching and demonstrations.
Normal Phase Chromatography (NPC)
Purpose is to separate molecules based on polarity; application includes separation of lipids, steroids, and vitamins.
Hydrophobic Interaction Chromatography (HIC)
Purpose is to separate proteins based on their hydrophobicity; application includes biochemistry for purifying proteins.
Size-Exclusion Chromatography (SEC)
Purpose is to separate based on molecular size; application includes biochemistry for protein purification and sizing.
Liquid Chromatography (LC)
Purpose is to separate mixtures with multiple components; application includes pharmaceutical industry for drug purification.
Flash Chromatography
Purpose is to purify compounds rapidly; application includes chemistry for quick purification of samples.
Affinity Chromatography
Purpose is to purify or concentrate a single substance from a mixture; application includes biotechnology for enzyme purification.
Thin-Layer Chromatography (TLC)
Purpose is to quickly and cheaply separate compounds; application includes organic chemistry for monitoring reactions.
Capillary Electrophoresis (CE)
Though not strictly chromatography, its purpose is to separate ionic compounds by their charge-to-size ratio; application includes biotechnology for DNA separation.
Gas Chromatography (GC)
Purpose is to separate volatile compounds; application includes forensics for analyzing substances in crime scenes.
Reverse Phase Chromatography (RPC)
Purpose is to separate molecules based on hydrophobicity; application includes analytical chemistry for detecting nonpolar compounds.
High-Performance Liquid Chromatography (HPLC)
Purpose is to separate, identify, and quantify each component in a mixture; application includes food industry for detecting contaminants.
Chiral Chromatography
Purpose is to separate enantiomers; application includes pharmaceutical industry for separating optically active isomers.
Supercritical Fluid Chromatography (SFC)
Purpose is to separate components that are not easily amenable to GC or HPLC; application includes pharmaceuticals for chiral separations.
Ion Exchange Chromatography
Purpose is to separate ions based on their charge; application includes water purification and separation of proteins.
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