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Electrophoresis Principles

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Colored substances mixed with samples in electrophoresis to track the progress of the molecule migration during the run.

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Capillary Electrophoresis

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An electrophoresis method where samples are separated within a small capillary tube, which allows for high resolution and faster analysis.

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Gel Electrophoresis

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A type of electrophoresis where biomolecules are separated by size as they migrate through a gel matrix.

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Electrical Field

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In electrophoresis, it is generated by applying a voltage across the gel, causing molecules to migrate.

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Voltage Gradient

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The drop in electrical potential per unit distance, controls the speed and efficiency of biomolecule separation in electrophoresis.

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DNA Ladder

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A mixture of known DNA fragment sizes used as a standard to estimate the size of DNA fragments in gel electrophoresis.

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Isoelectric Point

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The pH at which a molecule carries no net electric charge and does not migrate in an electric field, important in isoelectric focusing.

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Agarose Gel

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A porous gel matrix used in gel electrophoresis that's ideal for separation of larger DNA fragments.

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Cathode

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The negative electrode towards which positively charged particles migrate in electrophoresis.

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Electrophoresis

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A laboratory technique used to separate DNA, RNA, or protein based on size, charge, and other physical properties.

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Molecular Sieving

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The separation mechanism in gel electrophoresis where smaller molecules migrate faster through the gel matrix pores than larger ones.

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Anode

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The positive electrode towards which negatively charged particles migrate in electrophoresis.

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SDS-PAGE

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SDS Polyacrylamide Gel Electrophoresis, which denatures proteins and gives them a uniform negative charge for size-based separation.

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Polyacrylamide Gel

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A matrix used in gel electrophoresis suitable for separating small DNA or protein molecules.

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Buffer Solution

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A liquid that carries a current and maintains a stable pH during electrophoresis, facilitating the migration of molecules through the gel.

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